Coding

Part:BBa_K3699003:Design

Designed by: Haosheng Liu   Group: iGEM20_BUCT   (2020-10-21)


PylRS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 674
    Illegal NgoMIV site found at 1258
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PylRS: the pyrrolysyl-tRNA synthetase gene of Methanosarcina mazei (gene: MSMAC_3241, protein id: AKB73131.1), J23119 promoter



Source

PylRS designed according to Methanosarcina mazei.

References